Genetic improvement of rice for biotic and abiotic stress tolerance using molecular breeding
Scientific Team:
Dr. M. K. Modi, Professor & Head, Agricultural Biotechnology, AAU, Jorhat (PI).
Dr. S. K. Chetia, Chief Scientist, PBG, RARS, Titabar (PI).
Advisors: Dr. N. K. Singh, NIPB and Dr. Arvind Kumar, ICRISAT
Objectives:
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1) Identification of QTL/candidate gene for biotic/abiotic stress tolerance.
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Genome wide association studies (GWAS) for yield traits in Sali rice accessions.
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GWAS for drought tolerance traits in Ahu rice accessions.
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Bi-parental mapping of QTLs for submergence tolerance.
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High density mapping of QTLs associated with drought tolerance (Ranjit x Banglami).
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2) Introgression of QTLs/genes for biotic (Bacterial Leaf Blight, Blast) and abiotic (Drought) stress tolerance in local rice varieties.
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Introgression of Bacterial Leaf Blight and Blast resistance genes in rice.
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Molecular breeding for development of drought tolerant rice varieties.
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Salient accomplishment:
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60 significant associations detected using 50K SNPs for 11 agronomic traits, Phenotypic Variance (PV): 0-15%. (Verma et al, 2021, Genomics)
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30 QTLs (P.V:1.95-13.80%.) were identified for yield traits under drought (Banglami x Ranjit)
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38 QTLs (PV:1.97-12.42%) mapped for yield traits under drought in ‘Koni Ahu x Disang’
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Development of Bi-parental mapping population (F1s) for submergence tolerance
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Development of breeding population against BLB and Blast stress
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Two BC2F4 lines will be forwarded to AVT-NIL (drought) during 2022
Expected outputs :
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Gel-based functional markers for the SNPs associated with major yield traits
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Identification of Major QTLs/ candidate genes for drought and submergence stress
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BLB, Blast and drought tolerant rice varieties
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Introgression of drought tolerance in mega rice variety Ranjit Sub-1/Bahadur Sub-1.
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Development of biotic/abiotic stress tolerance in popular rice varieties of various North Eastern states i.e., Nagaland, Manipur, Tripura and Meghalaya.
![](../assets/img/rice_breeding/Picture1.jpg)
The drought tolerant BC2F4 lines selected for AICRP evaluation
![](../assets/img/rice_breeding/Picture2.png)
Phenotyping of advance breeding lines for drought tolerance